منابع مشابه
Concept of Enzyme Catalysis
A typical enzyme reaction runs through several steps. The structure of the enzymesubstrate intermediate in each step is reorganized to an optimal state to pass the next transition state. A very limited number of basic chemical catalytic principles are used in each transition state, and this is accomplished by a limited number of functional groups like amino acid side chains. Various methods of ...
متن کاملEnzyme dynamics during catalysis.
Internal protein dynamics are intimately connected to enzymatic catalysis. However, enzyme motions linked to substrate turnover remain largely unknown. We have studied dynamics of an enzyme during catalysis at atomic resolution using nuclear magnetic resonance relaxation methods. During catalytic action of the enzyme cyclophilin A, we detect conformational fluctuations of the active site that o...
متن کاملEnzyme Catalysis: Lessons from Stereochemistry
Stereoselectively labeled samples of E—2an Z—[9—2H,3H]— chorismate have been synthesized from Fand z-[3H, Hlphosphoen6lpyruvate by the enzymes of the shikimate pathway. The position of the tritium label at the 9—position of chorismate has been determined, from which it is evident that in the reaction catalyzed by 3-phosphoshikimate 1—carboxyvinyltransferase, the addition step has the opposite s...
متن کاملA perspective on enzyme catalysis.
The seminal hypotheses proposed over the years for enzymatic catalysis are scrutinized. The historical record is explored from both biochemical and theoretical perspectives. Particular attention is given to the impact of molecular motions within the protein on the enzyme's catalytic properties. A case study for the enzyme dihydrofolate reductase provides evidence for coupled networks of predomi...
متن کاملElectric Fields and Enzyme Catalysis.
What happens inside an enzyme's active site to allow slow and difficult chemical reactions to occur so rapidly? This question has occupied biochemists' attention for a long time. Computer models of increasing sophistication have predicted an important role for electrostatic interactions in enzymatic reactions, yet this hypothesis has proved vexingly difficult to test experimentally. Recent expe...
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ژورنال
عنوان ژورنال: Nature Materials
سال: 2012
ISSN: 1476-1122,1476-4660
DOI: 10.1038/nmat3483